Journal: eLife

Article Title: High-resolution mapping of fluoroquinolones in TB rabbit lesions reveals specific distribution in immune cell types

doi: 10.7554/eLife.41115

Figure Lengend Snippet: ( a–b–c ) Hematoxylin and Eosin (H and E) histology staining of lesions and surrounding lung tissue resected from rabbits that received a single dose of moxifloxacin (MXF) ( a ), levofloxacin (LVX) ( b ) or gatifloxacin (GTX) ( c ). N: necrotic core; white arrows: early caseating foci; yellow double arrows: cellular rim encompassing mostly lymphocytes, macrophages, foamy macrophages, interspersed epithelial cells and neutrophils. Panels d-e-f show the corresponding MALDI-MS ion maps of each drug in a tissue section adjacent to the one stained by H and E. Red arrows highlight the inner drug accumulation ring subtending the caseous core; yellow arrows highlight outer rings or pockets of higher drug abundance further outward from the core.

Article Snippet: At defined time points from 16 to 20 weeks post-infection, rabbits received a single dose of 100 mg/kg moxifloxacin (Chemieliva Pharmaceuticals, China), 75 mg/kg levofloxacin or 100 mg/kg gatifloxacin (Chem-Impex Intl, IL), formulated in 40% sucrose and PEG400 (90:10) by oral gavage.

Techniques: Staining

Journal: eLife

Article Title: High-resolution mapping of fluoroquinolones in TB rabbit lesions reveals specific distribution in immune cell types

doi: 10.7554/eLife.41115

Figure Lengend Snippet: ( A ) Relative quantitation of moxifloxacin (MXF), levofloxacin (LVX) and gatifloxacin (GTX), in caseum and uninvolved lung relative to the cellular rim, determined from MALDI ion maps using ImaBiotech Software Quantinetix (v 1.7, Loos, France). Areas of interest encompassing uninvolved lung, cellular rim, and caseum were delineated by first aligning and superimposing the MALDI MS image over the optical scan of the tissue. The MS image layer was made transparent and the areas were drawn based upon the optical scan. The average drug abundance [ion count of drug/ion count of internal standard] ratios between caseum and cellular rim or uninvolved lung and cellular rim are shown. To detect statistically significant differences between signal intensities in lung versus cellular lesion, and cellular lesion versus caseum, the data were analyzed using the Wilcoxon matched-pairs signed rank test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. ( B ) Absolute measurements of average fluoroquinolone pixel intensities, normalized to internal standard, which were used to calculate the intensity ratios shown in ( A ). ( C ) Spatial quantitation of MXF, GTX and LVX in and around necrotic granulomas at different times following a single oral dose in rabbits with active TB, relative to plasma concentrations at the time of lesion dissection. Uninvolved lung tissue, cellular rim and central caseum of necrotic lesions were isolated by laser-capture microdissection and analyzed by HPLC coupled to mass spectrometry (LC-MS/MS). Plasma concentrations were determined by standard LC-MS/MS.

Article Snippet: At defined time points from 16 to 20 weeks post-infection, rabbits received a single dose of 100 mg/kg moxifloxacin (Chemieliva Pharmaceuticals, China), 75 mg/kg levofloxacin or 100 mg/kg gatifloxacin (Chem-Impex Intl, IL), formulated in 40% sucrose and PEG400 (90:10) by oral gavage.

Techniques: Quantitation Assay, Software, Dissection, Isolation, Laser Capture Microdissection, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Biological psychiatry

Article Title: Dorsal amygdala neurotrophin-3 decreases anxious temperament in primates

doi: 10.1016/j.biopsych.2019.06.022

Figure Lengend Snippet:

Article Snippet: Antibodies Mouse monoclonal anti-NeuN (clone A60) MilliporeSigma Cat# MAB377; RRID_AB_2314889; Lot# 2806074 Antibodies Goat polyclonal anti-human NTF3 R&D Systems Cat# AF-267-NA; RRID_AB_354434;Lot# UY0614011 Virus Strains AAV5-CMV-NTF3 Vector Biolabs Chemicals, Peptides, and Recombinant Proteins Gadobenate dimeglumine (Multihance) Bracco Diagnostics Cat# 516413; NDC# 0270-5164-13 Commercial Assay Or Kit DPC Coat-a-count cortisol radioimmunoassay Siemens Cat# TK01; Lot# TKC01 1625;Cat# TK02; Lot# TKC02 1531; Cat# TK05; Lot# TKC05 1530 Commercial Assay Or Kit ImmuChem Cortisol Coated Tube radioimmunoassay kit MP Biomedicals Cat# 07–221105; Lot# FK1601,02,03 Commercial Assay Or Kit RNeasy Plus Mini kit Qiagen Cat# 74134 Commercial Assay Or Kit NuGEN Ovation RNAseq System v2 kit NuGEN Cat# 7102–32 Experimental Models: Organisms/Strains Macaca mulatta (RNA-seq; n= 46 male) Wisconsin National Primate Research Center; Harlow Center for Biological Psychology Experimental Models: Organisms/Strains Macaca mulatta (AAV5-NTF3; n=10 male) Wisconsin National Primate Research Center; Harlow Center for Biological Psychology Recombinant DNA pAAV-MCS Vector Biolabs Recombinant DNA Coding sequence of rhesus NTF3 This paper GenBank accession # {"type":"entrez-nucleotide","attrs":{"text":"XM_001118191","term_id":"297261604","term_text":"XM_001118191"}} XM_001118191 , bases 8 to 1033 Software; Algorithm RTHawk scanner interface HeartVista, Palo Alto, CA N/A Software; Algorithm VURTIGO toolkit Sunnybrook Health Sciences Centre; Toronto, Canada N/A Software; Algorithm Spamalize http://brainimaging.waisman.wisc.edu/õakes/spam/spam_frames.htm N/A Software; Algorithm ANTs http://stnava.github.io/ANTs/ N/A Software; Algorithm fmristat http://www.math.mcgill.ca/keith/fmristat/ N/A Software; Algorithm PerM Chen and Souaiaia, 2009 https://code.google.com/archive/p/perm/ Software; Algorithm RSeqFlow Wang et al., 2009 https://genomics.isi.edu/rnaseq Software; Algorithm SageRage – Exon Specific Alignment for Rhesus Souaiaia et al, 2018 https://github.com/tadesouaiaia/sage Software; Algorithm SageRage – Exon Quantficaton for Rhesus Souaiaia et al, 2018 https://github.com/tadesouaiaia/rage Other Custom Algorithms For Exon Specific Expression This paper http://aml31.genome.wustl.edu Other Web-resource for interrogating ancillary analyses.

Techniques: Plasmid Preparation, Recombinant, RIA Assay, Sequencing, Software, Expressing